NGAL (human, neutrophil gelatinase-associated lipocalin)
Mouse monoclonal antibody
Cat.No. HYB 211-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury (3).
Human recombinant HGAL in monomer and dimer forms adsorbed to aluminium hydroxide gel (4).
HYB 211-01 is specific for human NGAL. No cross-reaction with NGAL from other species has been detected.
Epitope specificity differs from that of HYB 211-02 and HYB 211-05.
HYB 211-01 reacts strongly with human NGAL. Strong reaction is seen when used as detection antibody in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. HYB 211-01 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections containing neutrophils. In Western blotting of SDS-PAGE gels, HYB 211-01 reacts with NGAL in both reduced and unreduced forms (4).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/16,000 | 4,3 |
| Immunoblotting | Yes | 1/500 | 4 |
| Immunohistochemistry | Yes | 4 |
1. Kjeldsen L, Johnsen AH, Sengelov H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Kjeldsen L, Koch C, Arnljots K, Borregaard N (1996) Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils. J Immunol Meth 198:155-164.
3. Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, Kelly C, Ruff SM, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P (2005) Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 365(9466):1231-1238.
4. Wagener G, Jan M, Kim M, Mori K, Barasch JM, Sladen RN, Lee HT (2006) Association between Increases in Urinary Neutrophil Gelatinase-associated Lipocalin and Acute Renal Dysfunction after Adult Cardiac Surgery. Anesthesiology 105:485-491.
5. Laurell H, Bouisson M, Berthelémy, Rochaix P, Déjan S, Besse P, Susini C, Pradayrol L, Vaysse N, Buscail L (2006) Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples. World J Gastroenterol 12:3344-3351.
The complete NGAL reference booklet can be found in the righthhand menu on this page.
Downloads
HYB 211-01.pdf (20.43 kB) HYB 211-01 specification sheet
NGAL human references.pdf (254.83 kB)
NGAL human references April 2008
CLI NGAL feature April 2007.pdf (2.25 MB)
CLI NGAL feature April 2007
MSDS
MSDS ABS Mabs Rev03.pdf (60.83 kB)
